منابع مشابه
Nuclear Binding of Progesterone in Chick Oviduct
1. Varied doses of labelled or unlabelled progesterone were injected into immature chicks which had previously been stimulated with oestrogen. The concentrations of nuclear bound [3H]progesterone were correlated with the effects of the hormone on endogenous RNA polymerase I and II activities in isolated oviduct nuclei. 2. The extent of nuclear localization of [H]progesterone in oviduct (a proge...
متن کاملProgesterone-binding Components of Chick Oviduct
The cytoplasmic progesterone-binding proteins of chick oviduct are shown to consist of two major 4 S fractions which occur in equal amounts. They are separated from each other by DEAE-cellulose chromatography. Purification of the two components, A and B, was about 800and 3000-fold, respectively, from estrogen-primed chick oviducts following ultracentrifugation, ammonium sulfate precipitation, g...
متن کاملProgesterone "receptors" in the cytoplasm and nucleus of chick oviduct target tissue.
This report demonstrates that the chick oviduct, a specific target organ for progesterone, contains both cytoplasmic and nuclear macromolecules which bind progestins. These binding molecules can be clearly distinguished from transcortin by centrifugation through sucrose gradients of low ionic strength and by agarose gel filtration. The cytoplasmic progesterone-binding molecules also bind 5-alph...
متن کاملInteraction of Estrogen and Progesterone in Chick Oviduct Development
The effects of estrogen and progesterone on the function of chick oviduct tubular gland cells have been studied. Such function, as measured by the increase in specific cell products such as lysozyme and ovalbumin, requires the continuous presence of estrogen or progesterone. Withdrawal of hormone results in a rapid cessation of function and an involution of the oviduct accompanied by rapid decr...
متن کاملFractionation of Chick Oviduct Chromatin
Chromatin isolated from several chick tissues was treated with micrococcal nuclease. A limited degree of tissue specificity of chromatinDNA resistance to nuclease digestion was observed. No difference in the extent of nuclease resistance of chromatin DNA was detected during oestrogen-induced oviduct differentiation. This suggested that the amount of non-histone chromosomal protein does not play...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1974
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(20)79734-6